Active soil substrates are commonly used to buffer pH (usually to slightly acidic conditions for plants or shrimps), but most shrimp soils also release some beneficial minerals and the soil also provides extra surface area for beneficial to colonize too.
If the source water has even lower pH than what the soil is supposed to buffer to, the soil doesn't buffer it back up.
When using R/O water, you are supposed to re-mineralize it to get the target trace elements, minerals, TDS and pH before water changes... it's not used directly to fill up a tank at its pure distilled form, as fishes and shrimps still need the essential trace elements and minerals in their water.
Topping up with R/O water is different as it's only in small amounts, the evaporated tank water has still left behind the elements and minerals, so it's okay to top it up with pure R/O water to maintain the TDS levels.
Co2 lowers pH, hence aeration actually tends to increases pH, this is because it promotes gas exchange and reduces Co2 levels.
Since you have a bare bottom tank and not using any active buffers, the pH dropping in your tank is mainly due to the effects and by-products of beneficial bacteria consuming and breaking down organic waste in the nitrogen cycle. The more organic waste being processed, the greater the cycle's effect is on lowering the water pH.
In your case most likely the pH decrease from the active nitrogen cycle has a greater effect than the pH increase from the aeration, hence overall pH still gradually lowers over time.
Bookmarks