Once you have fully cycled bio-media in a canister filter, if you increase the number of connected tanks, water volume or bio-load, the existing bacteria will grow to match it (assuming the bio-media volume/surface area is still sufficient for the added bio-load)... usually best to allow 2-3 days to let the system stabilize before adding more fauna progressively in stages.
The key is just to have ample bio-media and to allow time for the bacteria to grow to keep up with the new tank additions.












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