Hi! Welcome to AQ!
Concerning your question, there could be several reasons. So need yourself to sort out.
Are the harlequins new additions to the tank?
Any problem with other existing fish?
Anything you did that could have altered the water chemistry -- like major water change suddenly, washing the filter, adding chemicals/medications?
How about the temperature?
Notice anything unusual floating on the water surface?
In my case, there was once where my entire harlequin population (~20) was wiped out in my mature tank, leaving other fish intact. I could only think of the heat during that period and a sudden occurance of a dust-like floating algae in that tank. I used a surface skimmer to remove the floating stuff. But I did not replace the harlequin population after that to test the water. So I can't say for sure if my observations are correct.








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