Hi,
I am pretty sure C-500 will not have 4 trays. It will have at most 3 trays.
I suggest you use the following layout.
Tray 1 (the one which tank water will pass by first) Coarse filter medium, it should come in the box as a black piece of filter sponge, followed by finer filter wool.
Tray 2 and 3, bio media.
Bio media is basically any thing which beneficial bacterial can colonize and live on, I would suggest using ceramic ring (cheaper) or biohome (expensive but reputed to be effective in providing more living space for beneficial bacterial). Don't waste the limited area in your canister filter by adding things like japanese mat, bio balls which frankly takes up quite a fair bit of space.
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