I would suggest for you to use flipper instead of diffuser. Diffuser required a lots of pressure and your DIY CO2 may not generate sufficient pressure.
By the way, you can just sprinkle the yeast on top of the sugar water. What I mean is mixed the sugar and water well but don’t mix the yeast together. It take 24 hours before bubble start coming out from flipper, so it may take longer for diffuser.
Why don’t you use soft drink bottle? It was design for pressure, not sure if Gatorade bottle able to withstand the pressure and most importantly no leakage.
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