For canister, the sponge should be placed at the lowest tray since water is drawn in and get filtered upwards before leaving the outlet. So you would want the sponge at the lowest to seeve out the debris and let the upper tray do the biological thingy. For my case, inside my 3 tray canister, I have the 2 lower trays with sponge while my top tray is with ceramics and those bio star/ribs that came with the canister.








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