I'm using three canister filters in my 5 ft. Two of the canisters will be permanent while the third canister is a temp added to help establish my new tank.
Anyway, it would be best to run the two cansiters independently, i.e. 2 ins and 2 outs. Connecting them in series don't make much sense since it's double filtration on the same volume of water. Whereas running the canisters independently allow larger volume of water to be filtered.
Presently, one of my canister has mostly biological media while the other has nothing but filtering media.
Cheers,
Roy
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