Hi Jian Yang,
Sorry to hear about your fish, I can at least throw my 2cents in and give you some ideas.
The first thing that is strange is your nitrate is high and everything else is zero. Normally they all go together in the typical chain reaction. So first thought is fertilizer. Second would be that even strirring the substrate can release alot of Nitrate into the water column and cause spikes.
It really depends on what happened prior I guess.
Most fish can handle quite high nitrate levels, what really kills them is sudden changes in the levels. But some fish really have a problem with it.
But i am sure you know all of that. For nitrate poisoning though what most people dont know is that big water changes can be just as bad, we all panic and think "Oh my god" must change the water fast , but it can sometimes make more problems.
It might even not be the high nitrates, I noticed you said you used Pumice in your substrate, again a long shot but alot of the fish you mentioned are very sensitive to salts in the water, and it might be something as simple as the metallic salts from the pumice leaching into the water.
Just some ideas for you that are probably completely wrong.
Sorry about your fish.
Scott.
Thanks again,
Scott Douglass
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