I know it is not necessary or maybe harmful even to ON the CO2 for 24hrs, in the plant photosynthesis process, plant takes in O2 at night instead of CO2 or when the lights goes out. So this may suffocate your fishes if your plant start to flourish with excess CO2 and no O2. It should be alright if there are no fishes, the CO2 just goes to waste.
Hope this help. [:]








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