Place at outlet as you do not want anything to restrict flow going into your filter, it may result in damage to your pump head. Undissolved co2 may also cause airlock.
Bioballs are basically there to break the bubbles into finer bubbles so they dissolve more effectively. You can replace it with other materials, or if you have a large enough chamber (like the NA reactor), then you don't need anything inside the chamber.
Hope this helps.









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